The optical density (OD450) was measured at 450?nm utilizing a microtitre plate audience (BIO-Rad)
The optical density (OD450) was measured at 450?nm utilizing a microtitre plate audience (BIO-Rad). Next, the purified Compact disc133-scFv-1 50?l/well diluted 1:10 in 3% (w/v) BSA-PBS AKAP12 was added. over…