The cells were then set with cool 70% ethanol, washed with cool PBS, and stained with 50 g/ml propidium iodide (PI; Sigma-Adrich; Merck KGaA) in PBS, accompanied by the addition of 10 g/ml ribonuclease (Sigma-Adrich; Merck KGaA) and incubation for 4 h

The cells were then set with cool 70% ethanol, washed with cool PBS, and stained with 50 g/ml propidium iodide (PI; Sigma-Adrich; Merck KGaA) in PBS, accompanied by the addition…

COPD is due to prolonged contact with chemicals (3), specially cigarette (4), causing devastation of the tiny airways and lung parenchyma through chronic irritation (5) and other systems just like the increased variety of goblet cells, hyperplasia of mucoid glands and fibrosis (6)

COPD is due to prolonged contact with chemicals (3), specially cigarette (4), causing devastation of the tiny airways and lung parenchyma through chronic irritation (5) and other systems just like…