The cells were then set with cool 70% ethanol, washed with cool PBS, and stained with 50 g/ml propidium iodide (PI; Sigma-Adrich; Merck KGaA) in PBS, accompanied by the addition of 10 g/ml ribonuclease (Sigma-Adrich; Merck KGaA) and incubation for 4 h
The cells were then set with cool 70% ethanol, washed with cool PBS, and stained with 50 g/ml propidium iodide (PI; Sigma-Adrich; Merck KGaA) in PBS, accompanied by the addition…